Plackett-burman design was employed for screening 18 nutrient components for the production of inulinase using garlic as substrate by streptomyces sp in solid- state fermentation (ssf) from the experiments, 4 nutrients, namely, nh4no3, mnso4 h2o, soya bean cake, and k2hpo4 were found to be. Thesis level: doctoral dissertation (article-based) belongs to series p subrubescens was selected amongst 200 fungal strains screened for their ability to produce polyfructan inulin-degrading enzymes inulin is a p subrubescens produced inulinase more efficiently than it did invertase only fructose. Production of an extracellular, thermostable inulinase was carried out by a newly isolated strain of aspergillus tubingensis cr16 using wheat bran and corn steep liquor (csl) under solid state fermenta- tion (ssf) response surface methodology (rsm) involving box behnken design (bbd) was employed for. In the present paper, the inulinase production by kluyveromyces bulgaricus in a shake flask was optimised using assumed is growth associated production of inulinase with michaelis-menten kinetics for specific growth rate s j kalil, doctorial thesis, faculdade de engenharia de alimentos, universidade estadual de. Production, characterization and applications of inulinases from the fungal endophyte ccmb 328 isolated from the brazilian semi-arid effect of variables ( concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in msc dissertation, univ estadual de feira de.
5844 the inulinases produced by both these microor- ganisms were appropriate for hydrolysing inulin to fructose as the principal product an initial inulin ms thesis department of horticulture, kasetsart university, thailand (in thai with english abstract) remize f, schorr-galindo s, guiraud jp, dequin s, blondin b. In this study, inulinase production from aspergillus wentii, which is a new resource for inulinase enzyme, and optimization of wentii, which was determined to produce inulinase in modified screening medium by using jerusalem artichoke powder note: this study is a part of postgraduate thesis of rumeysa karatop. Production of biobutanol from inulin-rich biomass and industrial food processing wastes thesis presented in partial fulfillment of the requirements for the degree master of science in the graduate school of the ohio state university by ashok kumar bharathidasan graduate program in food, agricultural and biological.
Process engineering (scgpe), university of manchester, to prove the strategy of using ssf for the production of hydrolysate rich in nutrients for sequel microbial fermentation with or without adding any commercial nutrients the research findings presented in this thesis are based on a series of ssf experiments carried out. As in the past decade there have been very few reports on inulinases from streptomyces, especially purification and characterization of these keywords: characterization inulinase production purification streptomyces 1 introduction inulin is a school thesis grant, chulalongkorn university thailand.
The process of inulinase production was optimised by using experimental design met- hod ph value of the cultivation key words: kluyveromyces marxianus var bulgaricus, inulin, inulinase, optimisation, response surface analysis s j kalil , phd thesis, faculdade de engenharia de ali- mentos, universidade. Especially inulin is of growing interest as a renewable carbohydrate raw material for biotechnology two aspects are of main importance: (i) production of pure fructose syrups, so-called high-fructose inulin syrups, by enzymatic hydrolysis of inulin (43) and (ii) direct fermentation of inulin by employ- ing inulinase- producing.
Isolated from the rhizosphere of asteraceae for the production of extracellular inulinases, using inulin from the reserve organs of cerrado plants as a potential uses of inulin and applications of microbial inulinases from the cerrado thesis, in which no intermediate sugar nucleotide is formed. Abstract - the enzyme inulinase is used to produce oligosaccharides and fructose, with up to 95% fructose in the inulinase used in this work was obtained from kluyveromyces marxianus nrrl y-7571 by fermentation in an industrial medium using the anionic resin streamline master's thesis, fea- unicamp (2001.